Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences Lysozyme from chicken egg white, 12650-88-3, MFCD00131557, 1 g
Lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein, Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase. Lysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
Sigma Aldrich Fine Chemicals Biosciences DNase I, 100 mg
During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.
New England Biolabs, Inc. Shrimp Alkaline Phosphatase (rSAP) – 500 units
Shrimp Alkaline Phosphatase (rSAP) is a heat labile alkaline phosphatase purified from a recombinant source. rSAP is identical to the native enzyme and contains no affinity tags or other modifications. rSAP nonspecifically catalyzes the dephosphorylation of 5 and 3 ends of DNA and RNA phosphomonoesters. Also, rSAP hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). rSAP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5 protruding, 5 recessed and blunt ends. rSAP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. rSAP is completely and irreversibly inactivated by heating at 65C for 5 minutes, thereby making removal of rSAP prior to ligation or end-labeling unnecessary.
Enzyme Research Laboratories Inc Human a-Thrombin (Factor IIa),1000 U
Please use this part # if ordering a quantity of 1-9. If ordering qty 10 or more please use Fisher part # NC0704726.
Human a-Thrombin (Factor IIa),1000 U,Buffer Composition = 50 mM Sodium Citrate,0.2 M NaCl,0.1% PEG-8000,pH 6.5,Extinction Coefficient (1%) = 18.3,Molecular Weight = 37,000 daltons,Prepared from human Prothrombin by activation with Factor Xa
New England Biolabs, Inc. Thermolabile Exonuclease I – 15000 units
Thermolabile Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA (ssDNA) in the 3' to 5' direction.
New England Biolabs, Inc. Template Switching RT Enzyme Mix – 20 reactions
The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template. These non-templated nucleotides can anneal to a template switching oligo (TSO) with a known sequence, prompting the reverse transcriptase to switch template from RNA to the TSO. The resulting cDNA contains a known sequence (complementary to the sequence of the TSO) attached to the 3' end. This feature can be utilized in a variety of downstream applications, such as cDNA amplification, 5' RACE (rapid amplification of cDNA ends), and 2nd strand cDNA synthesis.
Sigma Aldrich Fine Chemicals Biosciences Accutase solution, MFCD01632905, 100mL
Accutase solution (in dulbecco′s phosphate-buffered saline (DPBS) without Ca++ or Mg++) contains proteolytic and collagenolytic enzymes for cell detachment. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.
Worthington Biochemical Corporation Trypsin, TPCK Treated, 100mg
A chromatographically purified, diafiltered, lyophilized powder that has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity [Kostka, V., and Carpenter, F.: JBC, 239, 1799 (1964)]. Store at 2-8°C. Protect from moisture.
Worthington Biochemical Corporation DNASE 1 100MG
Deoxyribonuclease I, 100 MG, Purified precrystalline DNase. A lyophilized powder. Store at 2-8degreeC., Source: Bovine Pancreas, Minimum Activity: =2,000 Kunitz units per mg dry weight
LGC GENOMICS LLC (Lucigen) Plasmid-Safe ATP-Dependent DNase, 1000 U @ 10 U/LDigest unwanted contaminating linear double-stranded DNA, without harming circular double-stranded DNA such as plasmids and fosmids.
Plasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations.
Macherey-Nagel rDNase Set
rDNase Set Recombinant DNase and rDNase Reaction Buffer, For RNA minipreparations with NucleoSpin kits
Worthington Biochemical Corporation OVOMUCOID TRYPSIN INHIBITOR 1G
Trypsin Inhbitor,Ovomucoid,1 gm,Source:Egg White,Minimum Activity:1 mg inhibits = 1.2 mg trypsin,A dialyzed,dried powder. Store at 2-8degreeC,Ovomucoids are the glycoprotein protease-inhibitors of avian egg white
LGC GENOMICS LLC (Lucigen) Terminator 5'-Phosphate-Dependent Exonuclease, 40U @ 1 U/LA processive 5 to 3 riboexonuclease that specifically digests RNA with 5-monophosphate ends only.
Terminator™ 5-Phosphate-Dependent Exonuclease is a processive 53 exonuclease that digests RNA that has a 5-monophosphate end. It does not digest RNA that has a 5-triphosphate, 5-cap or 5-hydroxyl group.
FUJIFILM Biosciences LYSYL ENDOPEPTIDASE 2AU
*Orders for this supplier are non-cancellable/non-returnable once they are processed.* Lysyl Endopeptidase, Pkg. Size:1 vial (2 AU), Preparation: A 10AU-vial contains Tris-HCl corresponding to 1mL of 2mm Tris HCl buffer (pH 8.0),MW: 27,000 (gel filtration); 30,000 (SDS electrophoresis)
LGC GENOMICS LLC (Lucigen) Baseline-ZERO DNase, 5000 UDigest large or small DNA molecules better than standard DNase I.
Baseline-ZERO™ DNase digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I.