Modifying Enzymes
LGC GENOMICS LLC (Lucigen) Ready-Lyse Lysozyme Solution, 4,000,000 UReady-to-use lysozyme solution to be used with our nucleic acid purification kits.
Ready-Lyse™ Lysozyme Solution is a recombinant lysozyme preparation, derived from a nonmammalian, nonavian source, that is useful for the lysis of Gram-negative (such as E. coli) and Gram-positive (such as Bacillus sp.) bacteria.
Sigma Aldrich Fine Chemicals Biosciences Accutase solution, MFCD01632905, 100mL
Accutase solution (in dulbecco′s phosphate-buffered saline (DPBS) without Ca++ or Mg++) contains proteolytic and collagenolytic enzymes for cell detachment. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.
New England Biolabs, Inc. Shrimp Alkaline Phosphatase (rSAP) – 500 units
Shrimp Alkaline Phosphatase (rSAP) is a heat labile alkaline phosphatase purified from a recombinant source. rSAP is identical to the native enzyme and contains no affinity tags or other modifications. rSAP nonspecifically catalyzes the dephosphorylation of 5 and 3 ends of DNA and RNA phosphomonoesters. Also, rSAP hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). rSAP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5 protruding, 5 recessed and blunt ends. rSAP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. rSAP is completely and irreversibly inactivated by heating at 65C for 5 minutes, thereby making removal of rSAP prior to ligation or end-labeling unnecessary.
Sigma Aldrich Fine Chemicals Biosciences Lysozyme from chicken egg white, 12650-88-3, MFCD00131557, 1 g
Lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein, Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase. Lysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
Worthington Biochemical Corporation PDS KIT 5/PK
Papain Dissociation System, Papain Vial, Code: PAP2, Vial reconstituted with 5mL of EBSS or equivalent yields a solution at 20U of papain permL in 1mm L-cysteine with 0.5mm EDTA, Store at 2 deg. to 8 deg.C, 5Vi
Accelagen TurboNuclease 250,000U, Endonuclease, 250 units/μl, 27 kDa, >99% purity, -20°C storage, 10-500 units/g cell paste, 25 units/mL in cell lysate, 5'-phosphorylated oligonucleotides 1-4 bases
TurboNuclease 250,000U, Endonuclease, 250 units/μl, 27 kDa, >99% purity, -20°C storage, 10-500 units/g cell paste, 25 units/mL in cell lysate, 5'-phosphorylated oligonucleotides 1-4 bases
FUJIFILM BIOSCIENCES INC LYSYL ENDOPEPTIDASE 2AU
*Orders for this supplier are non-cancellable/non-returnable once they are processed.* Lysyl Endopeptidase, Pkg. Size:1 vial (2 AU), Preparation: A 10AU-vial contains Tris-HCl corresponding to 1mL of 2mm Tris HCl buffer (pH 8.0),MW: 27,000 (gel filtration); 30,000 (SDS electrophoresis)
LGC GENOMICS LLC (Lucigen) Plasmid-Safe ATP-Dependent DNase, 1000 U @ 10 U/LDigest unwanted contaminating linear double-stranded DNA, without harming circular double-stranded DNA such as plasmids and fosmids.
Plasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations.
New England Biolabs, Inc. Hi-T4™ DNA Ligase – 100000 units
A thermotolerant variant of T4 DNA Ligase, Hi-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher temperatures than wild type T4 DNA Ligase. Hi-T4 DNA Ligase will join blunt end and cohesive end termini as well as repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids at temperatures as high as 50C.
New England Biolabs, Inc. Thermolabile Exonuclease I – 3000 units
Thermolabile Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA (ssDNA) in the 3' to 5' direction.
Sigma Aldrich Fine Chemicals Biosciences Liberase TL Research Grade, 10 mg
Liberase TL (Thermolysin Low) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.
Ankom Technology ALPHA AMYLASE 250ML
Alpha Amylase 250 ml. ANKOM FAA is a starch-hydrolyzing alpha-amylase enzyme with high heat and pH stability derived from a genetically modified strain of Bacillus licheniformis. Used for liquification of starch during Neutral Detergent Fiber Analysis.
Worthington Biochemical Corporation PAPAIN DISSOCIATION SYSTEM
Papain Dissociation System, 100mL of EBBS, 2degree to 8degreeC Storage Temp, 1BX
Sigma Aldrich Fine Chemicals Biosciences Neuraminidase (Sialidase) from Arthrobacter ureafaciens |
Neuraminidase (Sialidase) from Arthrobacter ureafaciens |
Sigma Aldrich Fine Chemicals Biosciences Amyloglucosidase from Aspergillus niger lyophilized powder, 30-60 units/mg protein (biuret), <=0.02% glucose | 9032-08-0 | MFCD00081350 | 100MG
Amyloglucosidase from Aspergillus niger lyophilized powder, 30-60 units/mg protein (biuret), <=0.02% glucose | 9032-08-0 | MFCD00081350 | 100MG